| dc.contributor |
Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig |
de_CH |
| dc.contributor |
Institut für Physikalische und Theoretische Chemie |
de_DE |
| dc.contributor.author |
Henze, B. |
de_DE |
| dc.contributor.author |
Bebber, C. |
de_DE |
| dc.contributor.author |
van den Heuvel, J. J. |
de_DE |
| dc.contributor.author |
Bilitewski, U. |
de_DE |
| dc.contributor.other |
Gauglitz, Günter |
de_DE |
| dc.date.accessioned |
2002-02-14 |
de_DE |
| dc.date.accessioned |
2014-03-18T10:09:47Z |
|
| dc.date.available |
2002-02-14 |
de_DE |
| dc.date.available |
2014-03-18T10:09:47Z |
|
| dc.date.issued |
2002 |
de_DE |
| dc.identifier.other |
099092271 |
de_DE |
| dc.identifier.uri |
http://nbn-resolving.de/urn:nbn:de:bsz:21-opus-4544 |
de_DE |
| dc.identifier.uri |
http://hdl.handle.net/10900/48326 |
|
| dc.description.abstract |
We present the detection of native mRNA using the BIACORE system. The influence of different probes and flow rates on the detection is shown and compared to the hybridisation of oligonucleotides. Probes for mRNA detection were chosen by calculations of secondary structures using energy minimizing criteria based on the algorithm of Zuker. Probe concentrations were optimised as well as the regeneration conditions for the sensor surface. The influence of the flow rate appeared to be more marked for mRNA than for oligonucleotide hybridisation. |
en |
| dc.language.iso |
en |
de_DE |
| dc.publisher |
Universität Tübingen |
de_DE |
| dc.rights |
ubt-nopod |
de_DE |
| dc.rights.uri |
http://tobias-lib.uni-tuebingen.de/doku/lic_ubt-nopod.php?la=de |
de_DE |
| dc.rights.uri |
http://tobias-lib.uni-tuebingen.de/doku/lic_ubt-nopod.php?la=en |
en |
| dc.subject.classification |
Biosensor , Messenger-RNS |
de_DE |
| dc.subject.ddc |
540 |
de_DE |
| dc.title |
Detection of mRNA using the BIACORE |
en |
| dc.type |
ConferenceObject |
de_DE |
| dc.date.updated |
2010-02-11 |
de_DE |
| utue.publikation.fachbereich |
Sonstige - Chemie und Pharmazie |
de_DE |
| utue.publikation.fakultaet |
7 Mathematisch-Naturwissenschaftliche Fakultät |
de_DE |
| dcterms.DCMIType |
Text |
de_DE |
| utue.publikation.typ |
conferenceObject |
de_DE |
| utue.opus.id |
454 |
de_DE |
| utue.publikation.source |
http://barolo.ipc.uni-tuebingen.de/biosensor2001/ |
de_DE |
