Detection of mRNA using the BIACORE

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Dokumentart: Teil einer Konferenzveröffentlichung
Date: 2002
Language: English
Faculty: 7 Mathematisch-Naturwissenschaftliche Fakultät
Department: Sonstige - Chemie und Pharmazie
DDC Classifikation: 540 - Chemistry and allied sciences
Keywords: Biosensor , Messenger-RNS
Other Contributors: Gauglitz, Günter
License: xmlui.dri2xhtml.METS-1.0.item-dc-rights_value_ubt-nopod
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We present the detection of native mRNA using the BIACORE system. The influence of different probes and flow rates on the detection is shown and compared to the hybridisation of oligonucleotides. Probes for mRNA detection were chosen by calculations of secondary structures using energy minimizing criteria based on the algorithm of Zuker. Probe concentrations were optimised as well as the regeneration conditions for the sensor surface. The influence of the flow rate appeared to be more marked for mRNA than for oligonucleotide hybridisation.

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