A Lipoxygenase Sensor for Essential Fatty Acid (EFA) Determination

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dc.contributor Ege University, Faculty of Science, Biochemistry Department, 35100-Bornova-Izmir/TURKEY de_CH
dc.contributor Institut für Physikalische und Theoretische Chemie de_DE
dc.contributor.author Önal, Secil de_DE
dc.contributor.author Timur, Suna de_DE
dc.contributor.author Akyilmaz, Erol de_DE
dc.contributor.author Telefoncu, Azmi de_DE
dc.contributor.other Gauglitz, Günter de_DE
dc.date.accessioned 2001-11-12 de_DE
dc.date.accessioned 2014-03-18T10:09:27Z
dc.date.available 2001-11-12 de_DE
dc.date.available 2014-03-18T10:09:27Z
dc.date.issued 2001 de_DE
dc.identifier.other 099529874 de_DE
dc.identifier.uri http://nbn-resolving.de/urn:nbn:de:bsz:21-opus-3688 de_DE
dc.identifier.uri http://hdl.handle.net/10900/48258
dc.description.abstract The essential fatty acids (EFAs) are a group of 12 compounds, but linoleic acid and a-linolenic acid are the only EFAs which are found in the diet, particularly in vegetable oils, in some abundance. The other on EFAs are either metabolic products of linoleic and a-linolenic acids or are found in the diet especially in marine foods, considerebly smaller amounts. The steadily growing knowledge about the correlation between the fatty acid composition of the diet and clinical disorders leads to a growing demand for a rapid and easy to use analytical device for fatty acid determination in foods. Up to date, the fatty acid composition of fats and oils is determined mainly by gas chromatography (GC), although GC is well established in lipid analysis and offers high sensitivities, it is still time consuming and laborious . In this study, we describe the development of enzyme sensors for the determination of w-3 and w-6 fatty acis from the mixture of EFAs. Since linoleic and a-linolenic acids show differences in first and second oxygenation activities, it is possible to analyse each of them in EFAs mixture. Lipoxygenases in general catalyse the oxygenation of polyunsaturated fatty acids containing a cis, cis-1,4-pentadiene system by molecular oxygen. The oxygen consumption due to the lipoxygenase catalysed oxygenation of EFAs monitored amperometrically. Lipoxygenase was immobilized on the surface of oxygen electrode by using different membrane systems. Each systems were compared with regard to linear ranges of the calibration plots, sensitivities, detection limits. Furthermore, optimization of working conditions (pH, temperature etc.) and stability tests were also studied. de_DE
dc.language.iso de de_DE
dc.publisher Universität Tübingen de_DE
dc.rights ubt-nopod de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_ubt-nopod.php?la=de de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_ubt-nopod.php?la=en en
dc.subject.classification Biosensor , Essentielle Fettsäuren , Omega-3-Fettsäuren de_DE
dc.subject.ddc 540 de_DE
dc.subject.other essential fatty acids en
dc.title A Lipoxygenase Sensor for Essential Fatty Acid (EFA) Determination de_DE
dc.type Sonstiges de_DE
dc.date.updated 2010-02-10 de_DE
utue.publikation.fachbereich Sonstige - Chemie und Pharmazie de_DE
utue.publikation.fakultaet 7 Mathematisch-Naturwissenschaftliche Fakultät de_DE
dcterms.DCMIType Text de_DE
utue.publikation.typ report de_DE
utue.opus.id 368 de_DE
utue.publikation.source http://barolo.ipc.uni-tuebingen.de/biosensor2001/ de_DE

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