The case for membrane-associated RNA-binding proteins in Saccharomyces cerevisiae

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dc.contributor.advisor Jansen, Ralf-Peter (Prof. Dr.) Syed, Muhammad Ibrahim 2019-09-12T08:30:37Z 2019-09-12T08:30:37Z 2021-08-21
dc.identifier.uri de_DE
dc.description.abstract The endoplasmic reticulum (ER) is the site of protein synthesis for secretory and membrane proteins. I aimed to identify RNA-binding proteins (RBPs) in yeast that associate with ER and to assess their role in mRNA localization and/or translation at ER. I identified several RBPs that partially colocalize with ER such as Khd1p, Mrn1p, Whi3p, Pbp2p, Slf1p, Ngr1p, and Puf1p. Only the deletion of Khd1p affected the localization of mRNAs such as MID2 and SLG1 to ER. Additionally, MID2 can localize to ER in a translation-independent manner. In contrast to the popular SRP model, RNA seq analysis in combination with qPCR and microscopic data revealed that mRNAs coding for nuclear-related proteins can also localize to ER. By deleting ER-associated RBPs such as Mrn1p, Whi3p, Pbp2p, Slf1p, and Ngr1p, I see an upregulation of mRNAs coding for nuclear-encoded MRPs and at the same time a loss in the protein levels of Mrpl39p, Mrpl44p, and Img2p. Furthermore, there is an absence of transcription for mitochondrial-encoded COX genes and the fivefold deletion strain cannot utilize nonfermentable carbon source such as glycerol. I propose that that Mrn1p, Whi3p, Pbp2p, Slf1p, and Ngr1p are involved in controlling the expression of nuclear-encoded MRPs. For ASH1 E3 LE recognition by She2p/She3p, the she2p tetramer uses two identical sets of amino acids (N36, R43, R52, K60, and R63) to contact RNA, while its protruding helix seems to be important for interaction with She3p. Mutations in the contact sites in She3p revealed that they were important for mRNA localization in vivo. en
dc.language.iso en de_DE
dc.publisher Universität Tübingen de_DE
dc.rights ubt-podno de_DE
dc.rights.uri de_DE
dc.rights.uri en
dc.subject.classification Nucleinsäuren de_DE
dc.subject.ddc 500 de_DE
dc.subject.other mRNA localization en
dc.subject.other Protein translation en
dc.subject.other Endoplasmic reticulum en
dc.title The case for membrane-associated RNA-binding proteins in Saccharomyces cerevisiae en
dc.type PhDThesis de_DE
dcterms.dateAccepted 2019-08-21
utue.publikation.fachbereich Biochemie de_DE
utue.publikation.fakultaet 7 Mathematisch-Naturwissenschaftliche Fakultät de_DE
utue.publikation.noppn yes de_DE


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