Short Protein Evolution Stories: Illuminating Aspects of MempromCC and Histidine Kinase Evolution

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dc.contributor.advisor Forchhammer, Karl (Prof. Dr.)
dc.contributor.author Karamichali, Ioanna
dc.date.accessioned 2018-11-22T10:26:33Z
dc.date.available 2018-11-22T10:26:33Z
dc.date.issued 2018-11-22
dc.identifier.other 513975543 de_DE
dc.identifier.uri http://hdl.handle.net/10900/84943
dc.identifier.uri http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-849432 de_DE
dc.identifier.uri http://dx.doi.org/10.15496/publikation-26333
dc.description.abstract Sequence-based bioinformatic analysis has long been a driving force of evolutionary studies, while experimental approaches offer new insights through mutagenesis, in vivo and in vitro evolution. Here, we employ both approaches to illuminate aspects of the evolution of two different protein families, the mempromCC protein family and the histidine kinase protein family. The mempromCC family comprises membrane-bound coiled-coil-containing proteins found mostly in mitochondria. Our bioinformatic analysis connects for the first time these proteins allowing their collective structural and functional analysis. While the mechanism of function remains elusive, these proteins have been shown to act as assembling factors of different proteins like the mitochondrial Ca2+ uniporter (MCU), cytochrome c and Photosystem I that are crucial for cell survival. Histidine kinase proteins typically function as multidomain proteins, comprising transmembrane sensor and cytoplasmic effector domains. In the osmoregulating histidine kinase EnvZ, signals are transmitted through the DHp (dimerization and histidine phosphotransfer) and the CA (catalytic and ATPbinding) domains: CA carries ATP and phosphorylates a histidine in DHp, which then transfers the phosphate group to downstream effectors, resulting in a modulated transcription of genes controlled by the ompC promoter. Based on the evolutionary traits observed for both DHp and CA an evolutionary scenario has emerged, in which the two domains were fused early in evolution building a histidine kinase from a simple ATP-binding element. To study this possibility, we produced a chimera, in which CA was replaced with DX, an artificial protein that was generated through in-vivo evolution by selecting for ATP binding affinity. Indeed, a DHp-DX fusion protein (DHp-DX1) showed a strong ATPase activity in vitro, that was not seen for DX alone. In vivo, DHp-DX1 increased the transcription of ompC-controlled genes compared to a phosphotransferasedeficient DHp-DX1(H15Q) mutant. Collectively, these findings support the modular evolution of the DHp-CA element and offer a proof of concept for primordial enzyme evolution. en
dc.language.iso en de_DE
dc.publisher Universität Tübingen de_DE
dc.rights ubt-podok de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_mit_pod.php?la=de de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_mit_pod.php?la=en en
dc.subject.classification Biologie , Evolution , Proteine , Coiled coil , Escherichia coli de_DE
dc.subject.ddc 500 de_DE
dc.subject.other protein evolution en
dc.subject.other histidine kinase en
dc.subject.other coiled coils en
dc.subject.other EnvZ en
dc.subject.other CA domain en
dc.subject.other artificial protein en
dc.subject.other de novo evolution en
dc.subject.other TCST systems en
dc.subject.other E. coli en
dc.subject.other signaling en
dc.subject.other DHp en
dc.subject.other DX protein en
dc.subject.other protein purification en
dc.subject.other functional analysis en
dc.subject.other ATPase en
dc.subject.other novel enzyme en
dc.title Short Protein Evolution Stories: Illuminating Aspects of MempromCC and Histidine Kinase Evolution en
dc.type Dissertation de_DE
dcterms.dateAccepted 2018-11-08
utue.publikation.fachbereich Biologie de_DE
utue.publikation.fakultaet 7 Mathematisch-Naturwissenschaftliche Fakultät de_DE
utue.publikation.source eLife 2016;5:e11861 de_DE

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