Tomato Receptors of the Bacterial Cold Shock Protein and the Plant Peptide Signal Systemin

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dc.contributor.advisor Felix, Georg (Prof. Dr.) Wang, Lei 2018-04-03T09:42:05Z 2018-04-03T09:42:05Z 2018-03-28
dc.identifier.other 507382846 de_DE
dc.identifier.uri de_DE
dc.description.abstract Plants have large families of plasma membrane localized receptors that can sense extracellular signals and initiate appropriate cytoplasmic responses. The csp22 peptide derived from bacterial cold shock protein (CSP) has been known as an immune elicitor for more than a decade, but the corresponding receptor has not been identified. Discovered more than a quarter-century ago as the first plant peptide hormone, systemin was shown to be critical for systemic wound response and anti-herbivore defense in tomato. The receptor for this peptide hormone also remains elusive, since the previously proposed receptor SR160 is a tomato homolog of the brassinosteroid receptor BRI1 and its role as systemin receptor could not be corroborated in later work. Our work started with the observation that the wild tomato Solanum pennellii, in contrast to the cultivated tomato Solanum lycopersicum, lacks responsiveness to csp22 and systemin, indicating natural variation in perception of both peptides. By making use of well-defined introgression lines of these two species, we mapped the genes responsible for csp22 and systemin sensing to a common genomic region on chromosome 3. This region contains around two dozen genes encoding potential cell surface receptors. Functional analysis of these individual receptor candidate genes expressed in leaves of young N. benthamiana plants revealed one leucine-rich repeat-receptor kinase (LRR-RK) that strongly enhanced responsiveness to csp22, which we named cold shock protein receptor (CORE), and two other closely related LRR-RKs that conferred sensitivity to systemin, which we named systemin receptor 1 and 2 (SYR1, SYR2). CORE and SYR1 showed high affinity and specificity for their respective ligands in receptor binding assays. They also proved functional when heterologously expressed in Arabidopsis cells. Furthermore, ectopic expression of CORE can confer Arabidopsis increased resistance to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Presence of SYR1, while not decisive for local and systemic wound responses as previously reported, is important for defense against insect herbivory in tomato plants. en
dc.language.iso en de_DE
dc.publisher Universität Tübingen de_DE
dc.rights ubt-podok de_DE
dc.rights.uri de_DE
dc.rights.uri en
dc.subject.classification Tomate , Rezeptor , Signal , Immunität <Medizin> de_DE
dc.subject.ddc 500 de_DE
dc.subject.other tomato en
dc.subject.other receptor en
dc.subject.other signal en
dc.subject.other immunity en
dc.subject.other bacterial cold shock protein en
dc.subject.other systemin en
dc.title Tomato Receptors of the Bacterial Cold Shock Protein and the Plant Peptide Signal Systemin en
dc.type PhDThesis de_DE
dcterms.dateAccepted 2018-03-21
utue.publikation.fachbereich Biochemie de_DE
utue.publikation.fakultaet 7 Mathematisch-Naturwissenschaftliche Fakultät de_DE
utue.publikation.source Published in Nature Plants 2, Article number: 16185 (2016); Published in Nature Plants volume 4, pages152–156 (2018) de_DE


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