Harnessing Human ADAR2 for Site-Directed RNA Editing

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dc.contributor.advisor Stafforst, Thorsten (Prof. Dr.)
dc.contributor.author Wettengel, Jacqueline
dc.date.accessioned 2016-10-17T09:11:27Z
dc.date.available 2016-10-17T09:11:27Z
dc.date.issued 2018
dc.identifier.other 507672240 de_DE
dc.identifier.uri http://hdl.handle.net/10900/72508
dc.identifier.uri http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-725085 de_DE
dc.identifier.uri http://dx.doi.org/10.15496/publikation-13919
dc.description.abstract The catalytic deamination of adenosine to inosine is an essential posttranscriptional RNA modification. Since Inosine is biochemically read as guanosine, site-specific A-to-I RNA editing results in substitution of single amino acids and can lead to changes in processing and regulation of transcripts. Because of the high potential for manipulating protein function and RNA processing, we are developing tools to re-direct RNA editing in a site-specific way by applying engineered SNAP-tagged deaminases. However, this strategy, as well as strategies by others, requires the presence of an engineered deaminase in the target tissue. With respect to this limitation we have now designed short guideRNAs that harness the endogenously expressed deaminase for site-directed RNA editing. We demonstrate the new method to edit user-defined targets with high specificity and yield. Editing was successfully optimized in vitro and in cell culture depending on a fully genetically encodable basis. This editing system has a high potential towards investigating and manipulating RNA processing and protein functions within current topics of biomedical issues. en
dc.language.iso en de_DE
dc.publisher Universität Tübingen de_DE
dc.rights ubt-podno de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=de de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=en en
dc.subject.classification Nucleinsäuren de_DE
dc.subject.ddc 500 de_DE
dc.subject.ddc 570 de_DE
dc.subject.other RNA editing en
dc.subject.other ADAR2 de_DE
dc.subject.other A-to-I editing en
dc.subject.other RNA Editierung de_DE
dc.title Harnessing Human ADAR2 for Site-Directed RNA Editing en
dc.type Dissertation de_DE
dcterms.dateAccepted 2016-04-19
utue.publikation.fachbereich Biochemie de_DE
utue.publikation.fakultaet 7 Mathematisch-Naturwissenschaftliche Fakultät de_DE

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