NMR studies of the archaeal exosome complex

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URI: http://hdl.handle.net/10900/72404
http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-724043
http://dx.doi.org/10.15496/publikation-13815
Dokumentart: PhDThesis
Date: 2016-10
Language: English
Faculty: 7 Mathematisch-Naturwissenschaftliche Fakultät
7 Mathematisch-Naturwissenschaftliche Fakultät
Department: Mathematisch-Naturwissenschaftliche Fakultät
Advisor: Sprangers, Remco (Dr.)
Day of Oral Examination: 2016-05-03
DDC Classifikation: 500 - Natural sciences and mathematics
Keywords: Strukturbiologie
Other Keywords: NMR
RNA-Abbau
Exosomkomplex
Proteindynamik
exosome
RNA degradation
protein dynamics
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Abstract:

This study is about structural studies of the exosome from Sulfolobus solfataricus. It includes dynamics analysis (by using Nuclear Magnetic Resonance) of the complex in absence and in presence of RNA. Additional biochemical experiments (degradation assays, fluorescence anisotropy) allowed further description of the interaction exosome-RNA.

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