A New Class of Molecules from Staphylococcus Species Affects Quorum Sensing and Growth of Gram-Negative Bacteria

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dc.contributor.advisor Götz, Friedrich
dc.contributor.author Chu, Ya-Yun
dc.date.accessioned 2014-10-08T07:19:10Z
dc.date.available 2014-10-08T07:19:10Z
dc.date.issued 2014
dc.identifier.other 415244005 de_DE
dc.identifier.uri http://hdl.handle.net/10900/56843
dc.identifier.uri http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-568439 de_DE
dc.description.abstract This thesis addressed bacterial communication between Gram positive and Gram negative bacteria. The knowledge that many pathogens rely on cell-to-cell communication mechanisms known as quorum sensing, opens a new disease control strategy: quorum quenching. While studying the interaction of staphylococci with Gram-negative bacteria, we came across another communication system in a Staphylococcus intermedius group (SIG) species which can act as a zoonotic pathogen. This is one of the rare examples where Gram-positive bacteria excrete two compounds in comparatively high amounts that suppress the quorum sensing signaling and inhibit the growth of a broad spectrum of Gram-negative beta- and gamma-proteobacteria. The excreted compounds, named yayurea A and B, were isolated from S. delphini and structurally characterized. In vitro studies with the Nacyl homoserine lactone (AHL) responding receptor LuxN of V. harveyi indicated that both compounds stimulated the LuxN-mediated phosphorylation of LuxU and caused the opposite effects with AHL. Furthermore, growth of yayurea A and B producing S. delphini is not suppressed by respiratory toxins when co-cultured with P. aeruginosa. Taken together, yayureas are presumably involved in self-protection and ensuring competitiveness in natural environments shared with Gram-negatives. In the second part of thesis, the role of protein A in Staphylococcus aureus adhesion to ethylene glycol coated surfaces was investigated. It was found that the coating materials, HS(CH2)11EG3OMe (EG3OMe) and poly-ethylene-glycol (PEG) prevented bacterial adhesion of S. aureus cells. However, pretreatment of EG3OMe and PEG coatings with r-globulins or serum strongly promoted adherence of S. aureus cell. Furthermore, the spa-deletion mutant S. aureus, lacking the IgG binding protein A, showed reduced adherence and pretreatment of S. aureus with serum significantly decreased adherence. These results suggested that r-globulins play a crucial role in promoting S. aureus cells adhesion by its IgG binding proteins. Particularly γglobulins bound to the coated surfaces thus mediating adherence of S. aureus via its protein A. Intercepting this adherence should prove to be useful in preventing biofilm formation. en
dc.language.iso en de_DE
dc.publisher Universität Tübingen de_DE
dc.rights ubt-podno de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=de de_DE
dc.rights.uri http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=en en
dc.subject.classification Staphylococcus de_DE
dc.subject.ddc 500 de_DE
dc.title A New Class of Molecules from Staphylococcus Species Affects Quorum Sensing and Growth of Gram-Negative Bacteria en
dc.type Dissertation de_DE
dcterms.dateAccepted 2014-08-04
utue.publikation.fachbereich Biologie de_DE
utue.publikation.fakultaet 7 Mathematisch-Naturwissenschaftliche Fakultät de_DE

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