Early detection of acute myocardial infarction with the new marker Fatty Acid-Binding Protein : rapid testing and diagnostic value

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Aufrufstatistik

URI: http://nbn-resolving.de/urn:nbn:de:bsz:21-opus-3617
http://hdl.handle.net/10900/48251
Dokumentart: Sonstiges
Date: 2001
Source: http://barolo.ipc.uni-tuebingen.de/biosensor2001/
Language: English
Faculty: 7 Mathematisch-Naturwissenschaftliche Fakultät
Department: Sonstige - Chemie und Pharmazie
DDC Classifikation: 540 - Chemistry and allied sciences
Keywords: Biosensor , Herzinfarkt
Other Contributors: Gauglitz, Günter
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Abstract:

There are several requirements for the early detection of AMI with biochemical markers: The biochemical marker should be: - Specific - Sensitive - Fast elevated after the clinical onset of the symptoms. The test should be: - Easy to perform - Give a fast result - Preferably bedside - Inexpensive Here, we demonstrate the biochemical characteristics of heart-type Fatty Acid-Binding Protein (FABP) in AMI and UAP patients. Also, we show some preliminary data on a new in-house made quantitative rapid test with simple test procedures. - Patients: 50 patients of the CCU of PWH are included in the study diagnosed with UAP or AMI. Eight blood samples per patient were taken at 0-72 hours after admission and analyzed with a laboratory ELISA test. - Rapid test procedure: 80 ml plasma or serum is added on the sample pad. Within 5 minutes, the strip can be read out with an optical reader (the PART from LRE, Munich, Germany). A release curve for AMI patients shows a significantly elevated FABP concentration already 1 hour after infarction, whereas for CPK, this is found after 3 hours. Comparing the ROC curve for patients arriving at the hospital and 1 hour later for FABP and CPK demonstrates the preference of FABP over CPK. The preliminary results of the rapid test show for selected plasma and serum samples a measuring range of 0-200 ng/ml FABP. The availability of a rapid bedside test for FABP enables faster diagnosis in patients with no clear ECG. This can be used for both inclusion or exclusion of AMI.

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