Abstract:
There are several requirements for the early detection of AMI with biochemical markers:
The biochemical marker should be:
- Specific
- Sensitive
- Fast elevated after the clinical onset of the symptoms.
The test should be:
- Easy to perform
- Give a fast result
- Preferably bedside
- Inexpensive
Here, we demonstrate the biochemical characteristics of heart-type Fatty Acid-Binding Protein (FABP) in AMI and UAP patients. Also, we show some preliminary data on a new in-house made quantitative rapid test with simple test procedures.
- Patients: 50 patients of the CCU of PWH are included in the study diagnosed with UAP or AMI. Eight blood samples per patient were taken at 0-72 hours after admission and analyzed with a laboratory ELISA test.
- Rapid test procedure: 80 ml plasma or serum is added on the sample pad. Within 5 minutes, the strip can be read out with an optical reader (the PART from LRE, Munich, Germany).
A release curve for AMI patients shows a significantly elevated FABP concentration already 1 hour after infarction, whereas for CPK, this is found after 3 hours.
Comparing the ROC curve for patients arriving at the hospital and 1 hour later for FABP and CPK demonstrates the preference of FABP over CPK.
The preliminary results of the rapid test show for selected plasma and serum samples a measuring range of 0-200 ng/ml FABP.
The availability of a rapid bedside test for FABP enables faster diagnosis in patients with no clear ECG. This can be used for both inclusion or exclusion of AMI.
