Multi-Parameter Fluorescence Detection at the Single-Molecule Level : Techniques and Applications

DSpace Repository

Show simple item record

dc.contributor MPI biophys. Chemie, Am Fassberg 11, 37077 Göttingen de_CH
dc.contributor Institut für Physikalische und Theoretische Chemie de_DE Eggeling, Christian de_DE Schaffer, Jörg de_DE Volkmer, Andreas de_DE Seidel, Claus de_DE Brand, Leif de_DE Jäger, Stefan de_DE Gall, Karsten de_DE
dc.contributor.other Gauglitz, Günter de_DE 2001-11-12 de_DE 2014-03-18T10:09:23Z 2001-11-12 de_DE 2014-03-18T10:09:23Z 2001 de_DE
dc.identifier.other 099445034 de_DE
dc.identifier.uri de_DE
dc.description.abstract Fluorescence-based assays using single-molecule techniques are evolving into a very important tool in science. These techniques include not only direct detection and analysis of single-molecule events, but also spectroscopic analysis through fluctuation methods such as FCS (fluorescence correlation spectroscopy) or FIDA (fluorescence intensity distribution analysis). What are the advantages of these techniques and why is fluorescence chosen as the readout? This article will give an overview of fluorescence techniques and applications of single-molecule detection and analysis. It is focused on the detection of fluorescence emitted from freely diffusing molecules in solution. A confocal microscope is described, which uses special electronics for detection and enables the observation of fluorescence emission from single molecules along with monitoring of various fluorescence parameters (MFD). In the first part, procedures such as the detection and identification of single molecules, the monitoring of rare events, the direct temporal observation of binding events and conformational dynamics, as well as the possibility of selective spectroscopy of molecular states are described. Industrial applications such as HTS are illustrated by a description of using fluctuation methods such as FCS and FIDA to resolve characteristic properties of biological assays, e.g., ligand-protein and -vesicle binding or enzymatic reactions. en
dc.language.iso en de_DE
dc.publisher Universität Tübingen de_DE
dc.rights ubt-nopod de_DE
dc.rights.uri de_DE
dc.rights.uri en
dc.subject.classification Biosensor , Fluoreszenzspektrometer , Einzelmolekülspektroskopie de_DE
dc.subject.ddc 540 de_DE
dc.subject.other Fluorescence Detection , single molecule spectroscopy en
dc.title Multi-Parameter Fluorescence Detection at the Single-Molecule Level : Techniques and Applications en
dc.type Teil einer Konferenzveröffentlichung de_DE 2010-02-10 de_DE
utue.publikation.fachbereich Sonstige - Chemie und Pharmazie de_DE
utue.publikation.fakultaet 7 Mathematisch-Naturwissenschaftliche Fakultät de_DE
dcterms.DCMIType Text de_DE
utue.publikation.typ conferenceObject de_DE 353 de_DE
utue.publikation.source de_DE


This item appears in the following Collection(s)

Show simple item record