Post-Translational Regulation of Bacterial Physiology by Ser/Thr Kinases HipA and HipH - A Mass Spectrometry Study

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Zitierfähiger Link (URI): http://hdl.handle.net/10900/161197
http://nbn-resolving.org/urn:nbn:de:bsz:21-dspace-1611977
http://dx.doi.org/10.15496/publikation-102529
Dokumentart: Dissertation
Erscheinungsdatum: 2025-01-24
Sprache: Englisch
Fakultät: 7 Mathematisch-Naturwissenschaftliche Fakultät
Fachbereich: Biologie
Gutachter: Macek, Boris (Prof. Dr.)
Tag der mündl. Prüfung: 2024-12-19
DDC-Klassifikation: 500 - Naturwissenschaften
570 - Biowissenschaften, Biologie
Freie Schlagwörter:
Phosphoproteome
Mass Spectrometry
Bacterial Kinases
Ser/Thr Kinases
Lizenz: http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=de http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=en
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Abstract:

Post-translational modifications (PTMs) play an indispensable role in the rapid execution of responses that regulate a number of biological functions. Protein Ser/Thr kinases (STKs) are key regulators of vital cellular processes, including antibiotic tolerance, metabolism, virulence, and stress response. Despite their importance, the molecular mechanisms and targets of STKs are underinvestigated, especially in the context of post-translational regulation in pathogenic bacteria. Antibiotic tolerance and persistence are a major contributor to the relapse of many chronic infections and frequently result in antibiotic overuse and the development of antibiotic resistance. One of the best-studied drivers of persistence is a Ser/Thr kinase HipA, first characterized in Escherichia coli. Multiple HipA-like kinases have been recently reported to be present in bacteria, including pathogens such as Klebsiella pneumoniae, but their functions remain poorly understood. In my thesis, I focused on elucidating the function of two such STKs, HipH (YjjJ) in E. coli and HipA in K. pneumoniae to understand their potential role in regulating cellular processes. To explore this, I applied state-of-the-art mass spectrometry-based quantitative phosphoproteomics to gain new insights into the functions and substrates of these kinases and fill crucial gaps in knowledge of bacterial physiology and pathogenesis. I first reviewed recent advances in quantitative phosphoproteomics to highlight the utility of LC-MS/MS technologies combined with quantitative proteomics strategies to investigate dynamic phosphorylation changes during various biological processes. I then applied this technology to study HipA-family Ser/Thr kinase HipH (YjjJ) in E. coli. Using quantitative phosphoproteomics based on stable isotope labeling by amino acids in cell culture (SILAC) and in vitro kinase assay, I demonstrated that HipH phosphorylates specific targets such as the ribosomal protein RpmE and the carbon storage regulator CsrA. Therefore, HipH plays an important role in regulating ribosome assembly, cell division, and central carbon metabolism, but it does not confer antibiotic tolerance like its homolog HipA. I have also shown that HipH cross-talks with other bacterial kinases, revealing a complex network of regulatory interactions. The final part of my work focused on Klebsiella pneumoniae, a major cause of antibiotic-resistant VIII nosocomial infections worldwide. I demonstrated that overproduced K. pneumoniae HipA (HipAkp) is toxic to both E. coli and K. pneumoniae, and this toxicity can be rescued by overproduction of the antitoxin HipBkp. Importantly, I showed that HipAkp overproduction leads to increased tolerance against ciprofloxacin, linking HipA activity to antibiotic persistence in this organism. Through proteome and phosphoproteome analyses, I confirmed that HipAkp has Ser/Thr kinase activity, auto-phosphorylates at S150, and shares multiple substrates with its E. coli counterpart. I performed a comprehensive analysis of the K. pneumoniae phosphoproteome with HipAkp overproduction to generate the largest dataset of phosphorylated proteins for this bacterium. Overall, my work provides an in-depth analysis of the roles of the two HipA-like kinases in antibiotic tolerance and metabolism, offering new insights into their functions and regulatory networks. These findings also provide a foundation for future research on post-translational regulation of bacterial physiology.

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