Development of Metabolomics Workflows in Clinical Bioanalysis by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

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URI: http://hdl.handle.net/10900/159741
http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-1597410
http://nbn-resolving.org/urn:nbn:de:bsz:21-dspace-1597412
http://nbn-resolving.org/urn:nbn:de:bsz:21-dspace-1597415
http://dx.doi.org/10.15496/publikation-101074
Dokumentart: PhDThesis
Date: 2025-12-31
Language: English
Faculty: 7 Mathematisch-Naturwissenschaftliche Fakultät
Department: Pharmazie
Advisor: Lämmerhofer, Michael (Prof. Dr.)
Day of Oral Examination: 2024-12-06
DDC Classifikation: 000 - Computer science, information and general works
License: http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=de http://tobias-lib.uni-tuebingen.de/doku/lic_ohne_pod.php?la=en
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Inhaltszusammenfassung:

Die Dissertation ist gesperrt bis zum 31. Dezember 2025 !

Abstract:

Throughout the recent years, the field of metabolomics has seen interest from the life science community, as it is able to provide valuable information about the quantitative and qualitative alterations of endogenous polar metabolites upon specific perturbations. Coupled with the substantial technological innovations in mass spectrometry (MS) over the last decades, MS-based metabolomics has attained the role of a valuable tool in bioanalysis and diagnosis, enabling us to detect the early manifestations of certain diseases and metabolic disorders and their progress. The aim of this thesis was to develop new methods and optimize existing workflows for metabolomics in clinical bioanalysis. On the one hand, the focus was to improve both targeted and untargeted assays, which allow broad metabolite profiling in each sample. Focus was laid upon the quantification capabilities of such assays, whilst at the same time allowing broad metabolite coverage. The developed methods were implemented in various types of biological matrix (cell culture, plasma and urine) and successfully displayed the desired outcome.

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