2026-09-18T17:38:44Zhttps://publikationen.uni-tuebingen.de/oai/requestoai:publikationen.uni-tuebingen.de:10900/1756192026-02-13T02:00:21Zcom_10900_42126col_10900_42133
Publikationssystem UB Tuebingen
advisor
Rapaport, Doron (Prof. Dr.)
author
Qian, Jiaxin
2026-02-12T13:29:01Z
2026-02-12
http://hdl.handle.net/10900/175619
Mitochondria are essential organelles in almost all eukaryotic cells. They are the powerhouses
of the cell, in charge of the production of ATP. In addition, they are also involved in calcium
signaling, immune responses, and programmed cell death (apoptosis), all while producing
reactive oxygen species (ROS). Their dysfunction can lead to various diseases and aging
effects. There are more than 1000 different proteins in mitochondria, with 99% of them encoded
by the nuclear genome. They are translated by cytosolic ribosomes, transported to and finally
imported into mitochondria. The ribosome-associated complex (RAC) is required for cotranslational folding, chaperone coordination, protein quality control and stress response. It
consists of an Hsp40 family protein (Zuo1) and a non-typical Hsp70 family protein (Ssz1) in
yeast.
This study mainly focuses on:
(1) How does Zuo1 influence the biogenesis of mitochondrial outer membrane proteins?
(2) What are the potential genetic interactions between Zuo1 and the mitochondrial protein
import receptors Tom70/Tom71 under normal and heat stress conditions?
To address these questions, I analyzed yeast cells that harbor single or multiple deletions in
the genes of interest, namely ZUO1, TOM70 and/or TOM71. The results mainly demonstrated
that: First, the absence of Zuo1 affects growth differently under normal and heat-stress
conditions when Tom70 and Tom71 are lost. Second, the absence of Zuo1 changes the
proteostasis of cells lacking Tom70/71. This study introduces a novel paradigm in which RAC
functions as a stress-controlled regulatory component of the cytosolic translation machinery.
en
ubt-podno
The role of Zuo1 in mitoprotein-induced stress
PhDThesis
URL
https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/175619/1/The%20thesis%20of%20Jiaxin%20Qian.pdf
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The thesis of Jiaxin Qian.pdf
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https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/175619/4/The%20thesis%20of%20Jiaxin%20Qian.pdf.txt
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The thesis of Jiaxin Qian.pdf.txt