2026-09-18T17:08:21Zhttps://publikationen.uni-tuebingen.de/oai/requestoai:publikationen.uni-tuebingen.de:10900/1733052025-12-21T00:04:09Zcom_10900_42126col_10900_42133
Publikationssystem UB Tuebingen
advisor
Schenke-Layland, Katja (Prof. Dr.)
author
Marsall, Patrick
2025-12-18T12:19:45Z
2026-11-30
http://hdl.handle.net/10900/173305
With seasonal epidemics each year and several global pandemics, respiratory viruses
represent a significant global health burden. Respiratory syncytial virus (RSV) and Influ-
enza viruses are of particular concern as they have a severe impact on vulnerable popu-
lations such as infants and the elderly and can trigger global pandemics through antigenic
drift or zoonotic transmission. The disruption of viral seasonality caused by the SARS-
CoV-2 pandemic has hampered continuous epidemiologic surveillance and highlighted
the need for reliable serologic tools to assess immunity and infection status at the popu-
lation level in times of limited clinical surveillance.
The aim of this thesis was to develop and validate two antibody binding multiplex immu-
noassays, RSV MIA and FLU MIA, that enable sensitive and subtype-resolved detection of
virus-specific antibody responses in both serum and other sample matrixes. These as-
says were then used to generate population-level serological data on RSV and Influenza
immunity and infections within a longitudinal retrospective cohort study. The assays were
then also used to investigate vaccine-induced immune responses, such as Influenza an-
tibodies produced in an organ-on-chip model system. To achieve this, I developed and
validated the RSV MIA and the FLU MIA according to EMA and FDA guidelines, enabling
robust high-throughput quantification of antigen-specific antibody titers. To determine
assay performance and to understand the immune response to an RSV/Influenza infec-
tion, both assays were clinically validated using samples from human challenge studies.
When used in a longitudinal population cohort study, RSV MIA could be used to identify
seasonal RSV infections, often including the viral subtype. FLU MIA showed high sensitiv-
ity in detecting broad and strain-specific humoral responses after both influenza vaccina-
tion and seasonal infection, including seasonal patterns induced by circulating influenza
strains between years. In the organ-on-chip system, FLU MIA successfully quantified an-
tigen-specific antibodies in response to vaccine administration, confirming the ability of
lymphoid tissue to elicit measurable immune responses ex vivo. Taken together, these re-
sults underscore the versatility of both assays for seroepidemiologic studies and vaccine
testing platforms. By enabling detailed analyses of infection- and vaccine-induced hu-
moral immunity, these assays provide a valuable foundation for future epidemiologic sur-
veillance and next-generation vaccine evaluation.
Dissertation ist gesperrt bis 30. November 2026 !
en
ubt-podno
Post-Pandemic Serosurveillance of RSV and Influenza: Development and Application of Multiplex Immunoassays
PhDThesis
URL
https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/173305/2/PhDThesisMarsallPatrick.pdf
File
MD5
ca651c65c57dcac801ae77402e413690
4111588
application/pdf
PhDThesisMarsallPatrick.pdf
URL
https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/173305/3/PhDThesisMarsallPatrick.pdf.txt
File
MD5
5c8484b1a4c1205bfe7036f1f13a8bd1
293411
text/plain
PhDThesisMarsallPatrick.pdf.txt