2026-09-18T17:32:31Zhttps://publikationen.uni-tuebingen.de/oai/requestoai:publikationen.uni-tuebingen.de:10900/1432372025-06-20T10:31:41Zcom_10900_42126col_10900_42133
Publikationssystem UB Tuebingen
advisor
Stegmann, Evi ( Apl. Prof. Dr.)
author
Hernández Pérez, Naybel
2023-07-10T08:46:29Z
2025-06-20
http://hdl.handle.net/10900/143237
Zinc is a crucial trace element for bacteria and plays an essential role in several physiological processes, including DNA replication and metabolism. Bacteria
have evolved various mechanisms to acquire zinc from the environment, such as
the production of zincophores. One example is the zincophore [S,S]-EDDS produced by Amycolatopsis japonicum. [S,S]-EDDS is an isomer of ethylenediamine tetraacetate (EDTA), which is widely used in industry. However, unlike EDTA, [S,S]-EDDS is biodegradable, making it a promising alternative with a favorable environmental profile. Besides its application in various industrial processes, [S,S]-
EDDS is also interesting for agriculture. These two aspects were investigated in
the present work.
(i) [S,S]-EDDS has demonstrated efficacy as a Fe2+ and Zn2+ fertilizer, but its short
degradation time limits its long-term impact. To overcome this limitation, a promising approach is to use the [S,S]-EDDS producer A. japonicum as a biofertilizer.
Continuous production of [S,S]-EDDS would avoid the need for multiple applications of [S,S]-EDDS. The experiments carried out with Phaseolus vulgaris cv. Black
pole in calcareous soil showed that the use of A. japonicum as a biofertilizer, however, did not lead to any improvement in plant growth or microelement concentration.
(ii) For the use of [S,S]-EDDS as a chelating agent in various industrial applications, the yield of [S,S]-EDDS in A. japonicum needs to be improved. A prerequisite for this is the understanding of the biosynthesis of [S,S]-EDDS. Using a wide
range of methods (biochemistry, genetics, and labeling studies) it was confirmed
that L-aspartic acid and oxaloacetic acid are precursors for [S,S]-EDDS biosynthesis. In addition, ((S)-2-amino-2-carboxyethyl)-L-aspartic acid (ACEAA) was identified for the first time as a biosynthetic intermediate. Furthermore, the results
strongly indicated that the previously proposed precursor, L-diaminopropionic
acid (L-DAPP), is not involved in the biosynthesis, suggesting a novel biosynthetic pathway for [S,S]-EDDS. Genetic strategies were used to redirect the metabolic flux
towards the desired metabolites to further optimize [S,S]-EDDS yields. To prioritize target genes for metabolic engineering, the bioinformatic tool Secondary Metabolite Transcriptomic Pipeline (Sema-Trap) was used for RNA-Seq-based transcriptome analysis. This identified bldCAj, lacIAj and gltsAj as target genes for engineering in A. japonicum. Overexpression of these genes resulted in a 3-fold increase in [S,S]-EDDS production compared to A. japonicum wild type. Taken together, these findings provide the potential for further progress in optimizing [S,S]-EDDS production.
en
ubt-podno
Investigation on [S,S]-EDDS biosynthesis and its applications
PhDThesis
URL
https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/143237/2/05.07.2023_Dissertation__Naybel_Hernandez_Perez.pdf
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05.07.2023_Dissertation__Naybel_Hernandez_Perez.pdf
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https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/143237/3/05.07.2023_Dissertation__Naybel_Hernandez_Perez.pdf.txt
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