2026-09-18T17:49:26Zhttps://publikationen.uni-tuebingen.de/oai/requestoai:publikationen.uni-tuebingen.de:10900/1144602023-03-31T07:57:41Zcom_10900_42126col_10900_42130
Publikationssystem UB Tuebingen
advisor
Zeck, Günther (Prof. Dr.)
author
Corna, Andrea
2021-04-23T10:57:47Z
2023-03-31
http://hdl.handle.net/10900/114460
Millions of people worldwide suffer from retina degeneration pathologies.
To this day the only clinically approved treatment for these pathologies
are retina implants. However, state of the art implants fail to rescue vision
beyond the level of legal blindness. New stimulation strategies are therefore
required to improve retina implant performances, and new treatments must
be developed to avoid or delay retina degeneration. In this framework,
microelectrode arrays represent an optimal platform to investigate vision
restoration strategies in vitro.
In the dissertation three different studies are included, with the goal of
evaluating current approaches to vision restoration and to investigate new
solutions to improve artificial vision. (1) I investigated subretinal electrical
pulsatile stimulation of photoreceptor-degenerated mouse retina (rd10 strain)
using the retina implant ALPHA AMS R3 chip. I demonstrated efficient and
safe stimulation with a single 30 μm electrode. I evaluated the charge threshold
dependency with electrode size and reported non-monotonic stimulus
response relationship. (2) Using a high-density CMOS based microelectrode
array, I evaluated spatial and contrast resolution obtained by sinusoidal
epiretinal stimulation. I demonstrated reliable and continuous stimulation
without fading and axonal stimulation. Using a logistic regression model
to analyze RGCs responses with simple shapes stimulation, I demonstrated
high accuracy discrimination of spatial object displacement of 32 μm and
artificial contrast level of 10%. (3) Evaluating light responsiveness of organotypic
retina culture, I contributed to the assessment of the efficacy of HDAC
inhibition to improve cone survival in retinitis pigmentosa.
en
ubt-podno
Assessment of vision restoration strategies in ex vivo photoreceptor-degenerated mouse retina using microelectrode array
PhDThesis
URL
https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/114460/2/Dissertation_Andrea_Corna.pdf
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https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/114460/3/Dissertation_Andrea_Corna.pdf.txt
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Dissertation_Andrea_Corna.pdf.txt