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oai:publikationen.uni-tuebingen.de:10900/1109722021-01-14T13:14:47Zcom_10900_42126col_10900_42133
Publikationssystem UB Tuebingen advisor Heilbronner, Simon (Dr. rer. nat.) author Müller-Jochim, Angelika 2020-12-29T08:20:03Z 2020-12-22 1744692866 http://hdl.handle.net/10900/110972 Gram-positive cocci are a major cause of healthcare associated infections. Staphylococcus aureus and Stapylococcus lugdunensis represent prominent species. Additionally, Gram-negative bacteria as Pseudomonas aeruginosa and Escherichia coli frequently cause infections. With the emerge of multi drug resistant strains, the development of new antibacterial agents against Gram-positive and Gram-negative pathogens becomes increasingly urgent. To maintain proliferation, bacteria need to adapt to different parameters as e.g. varying nutrient availability. During invasive disease, the access to trace nutrients such as iron (Fe) is actively limited by the host. This strategy is referred to as “nutritional immunity”. Besides trace metals like Fe, several metabolites as e.g. the amino acid methionine, are essential for bacterial growth and their availability in the human host is scarce. During infection bacteria either need efficient acquisition systems or must rely on the de novo synthesis of such nutrients. Here we provide insight into the unique role of an iron regulated ABC transporter from the energy-coupling factor type (ECF) in S. lugdunensis which is involved in heme acquisition. We showed that the Lha transporter is specific for heme and recombinant substrate-specific protein LhaS accepts heme from various hemoproteins. By creating isogenic mutants and recombinant expression of Lha we showed that its function is independent of the well-studied heme acquisition system Isd and allows usage of human cells as a source of iron. Our investigations revealed a new strategy of nutritional heme acquisition to overcome host-induced iron limitation. Additionally we investigated the importance of the methionine biosynthesis pathway for bacterial growth and biofilm formation. We found methionine auxotrophic mutants of S. aureus, E. coli and P. aeruginosa to depend on exogenous concentrations of methionine exceeding those reported for human serum. Growth characteristics and biofilm formation was impaired in auxotrophic strains of S. aureus and P. aeruginosa. Our studies suggest primary metabolic pathways as interesting targets for antimicrobial therapy. en ubt-podok Nutritional limitation of iron and methionine. Physiological consequences and new evasion strategies in staphylococci PhDThesis URL https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/110972/2/Dissertation%20Angelika%20M%c3%bcller-Jochim%20merged%20FINAL.pdf File MD5 8d5849d40729b8d206cfae59afa531de 3013710 application/pdf Dissertation Angelika Müller-Jochim merged FINAL.pdf URL https://publikationen.uni-tuebingen.de/xmlui/bitstream/10900/110972/4/Dissertation%20Angelika%20M%c3%bcller-Jochim%20merged%20FINAL.pdf.txt File MD5 6e982ca5a0865d904e8be5cdb3f0b4af 243993 text/plain Dissertation Angelika Müller-Jochim merged FINAL.pdf.txt